Polymer supported synthesis of oligonucleotides by a phosphotriester method.
نویسندگان
چکیده
A new polymer supported synthesis of deoxyribooligonucleotides which can be adapted to automation is described. The method is based on the elongation of an oligomer chain in the 5'- to 3' -end direction using the modified phosphotriester chemistry. The approach is exemplified by the synthesis of a nonanucleotide d(TTCGTCTTG).
منابع مشابه
New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.
A rapid and convenient method for the synthesis of deoxyribooligonucleotides has been developed using the phosphotriester approach. The advantage of this methodology for work in solution was successfully demonstrated in synthesis of a number of DNA fragments up to 32-long. Adaptation of the presented method to solid-phase synthesis allows a pentadecamer to be assembled in 4-5 hours using dinucl...
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عنوان ژورنال:
- Nucleic acids research
دوره 10 20 شماره
صفحات -
تاریخ انتشار 1982